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Vinculin Antibody

CAT: 0013-GTR18575199Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18575199Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa) . Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
UniProt
P18206
Reactivity
Human
Immunogen
Recombinant full-length human Vinculin protein was used as the immunogen for the Vinculin antibody.
Clonality
Monoclonal
Clone
VCL/3617
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Vinculin antibody should be determined by the researcher.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · P18206

Vinculin

VINC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P18206
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VCL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q16450, Q5SWX2, Q7Z3B8, Q8IXU7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationLipoproteinPalmitatePhosphoprotein

Molecular function

Actin-binding

Coding sequence diversity

Alternative splicing

Disease

CardiomyopathyDisease variant

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCell projectionCytoplasmCytoskeletonMembrane

Domain

Repeat

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