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Melan-A Antibody

CAT: 0013-GTR18575769Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18575769Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1/Melan-A is present in melanosomes and endoplasmic reticulum. This mAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
UniProt
Q16655
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this Melan-A antibody.
Clonality
Monoclonal
Clone
SPM555
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the Melan-A antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · Q16655

Melanoma antigen recognized by T-cells 1

MAR1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16655
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MLANA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MART-1
EC number
—
Processing
—
Secondary accessions
Q6ICU4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Endoplasmic reticulumGolgi apparatusMembrane

PTM

Phosphoprotein

Domain

TransmembraneTransmembrane helix

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