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Fibronectin Antibody

CAT: 0013-GTR18575383Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18575383Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibronectin is a dimeric glycoprotein of 440kDa which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This mAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin.
UniProt
P02751
Reactivity
Human
Immunogen
Purified human fibronectin was used as the immunogen for this Fibronectin antibody.
Clonality
Monoclonal
Clone
SPM539
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · P02751

Fibronectin

FINC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02751
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
FN
EC number
—
Processing
Precursor
Secondary accessions
B7ZLF0, E9PE77, E9PG29, O95609, O95610, Q14312, Q14325, Q14326, Q17RV7, Q53S27, Q564H7, Q585T2, Q59EH1, Q60FE4, Q68DP8, Q68DP9, Q68DT4, Q6LDP6, Q6MZS0, Q6MZU5, Q6N025, Q6N0A6, Q7Z391, Q86T27, Q8IVI8, Q96KP7, Q96KP8, Q96KP9, Q9H1B8, Q9HAP3, Q9UMK2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Acute phaseAngiogenesisCell adhesionCell shape

Coding sequence diversity

Alternative splicing

Disease

Disease variantDwarfism

PTM

Disulfide bondGlycoproteinIsopeptide bondOxidationPhosphoproteinPyrrolidone carboxylic acidSulfation

Cellular component

Extracellular matrixSecreted

Molecular function

Heparin-binding

Domain

RepeatSignal

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