P27Kip1 Antibody

CAT: 0013-GTR18575350Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18575350Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.

UniProt

P46527

Reactivity

Human

Immunogen

Mouse p27Kip1 protein was used as the immunogen for this p27 antibody.

Clonality

Monoclonal

Clone

SPM348

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The optimal dilution of the p27Kip1 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, kappa

UniProtKB · P46527

Cyclin-dependent kinase inhibitor 1B

CDN1B_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P46527
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CDKN1B
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: Q16307, Q5U0H2, Q9BUS6

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell cycle

Cellular component

CytoplasmEndosomeNucleus

PTM

PhosphoproteinUbl conjugation

Molecular function

Protein kinase inhibitor

Disease

Tumor suppressor

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