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Pro-MMP-8, human

CAT: 0026-7784-5Size: 5 µgDry Ice: YesHazardous: No
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CAT#:0026-7784-5Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human neutrophil collagenase (HNC) has been purified from extracts of fresh and outdated buffy coats and from exudates of phorbol myristate acetate-stimulated neutrophils. The MMP-8 present in the starting material can either be latent or active, or have an app. relative molecular mass of 75-kDa and/or 58-kDa. The rather complex pattern of activation of the latent 58-kDa and 75-kDa species by trypsin, organomercurials and oxidants has been investigated. MMP-8 was shown to preferentially hydrolyze type I over type II, and type III collagens in solution and to be a glycoprotein that contains complex N-linked oligosaccharides leading to multiple forms of MMP-8 in SDS-PAGE. The action of endoglycosidase on the latent 58-kDa form produces 42/40-kDa species (Gao et al. 1992, Mallya et al. 1990). This indicates that MMP-8 is an N-linked, complex glycoprotein that appears to be glycosylated at multiple sites.
Shipping Conditions
dry ice
Storage Conditions
-80°C
Shelf Life
12 months

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