PCR-Salmonella-Listeria Detection Kit

PCR-Salmonella-Listeria Detection Kit

• Description: The PCR-Salmonella-Listeria Detection kit is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp and/or Listeria monocytogenes by the real-time PCR method (multiplex PCR). The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, mgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM (Listeria) and HEX fluorochrome (Salmonella), whereas the probe for the detection of IAC is labelled with the CY5 fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.
• Reactivity: Listeria monocytogenes, Salmonella
• Applications: Real-time PCR
• Detection Method: Real-time PCR
• Features & Benefits: Easy and Ready-to-use Kit., Detection: probe labelled with fluorescent dyes– Salmonella: HEX-BHQ1; Listeria: FAM-BHQ1a; IAC:CY5-BHQ2., Fast: results in less than 24 hours with minimal handling., Simultaneously detect and differentiate Salmonella spp. and Listeria monocytogenes in the same tube., Amplification limit: 10 UG per reaction (100%)., Inclusivity: Salmonella: Positive in 30 reference strains of food origin from the Spanish Type Culture Collection (CECT). Listeria: Positive in 48 reference strains of L. monocytogenes (serovars 1 to 7) from different collections., Reliable and highly specific Results., Exclusivity: Salmonella: Tested with 29 nontarget strains. Listeria: 100% Tested with 94 nontarget strains composed of 51 strains of Listeria nomonocytogenes and 43 strains of non-Listeria., Quantification Dynamic range: 6 logs., Quantification limit: 20 UG per reaction (100%).
• Shipping Conditions: Dry Ice
• Storage Conditions: -20°C
• Shelf Life: 12 months