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TEV Protease Activity Assay Kit (Fluorometric)

CAT: 0026-K842-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K842-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TEV Protease (EC: 3.4.22.44, Tobacco Etch Virus protease) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s TEV Protease Activity assay kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). This assay kit is simple, rapid and can detect TEV Protease activity as low as 50 ng/well in viral lysates and purified protein samples.
Reactivity
Viral lysates
Applications
• Detection of TEV Protease activity in viral lysates • Determine activity of purified TEV Protease
Detection Method
Fluorescence (Ex/Em = 490/560 nm)
Features & Benefits
Simple, rapid assay Can detect as low as 50 ng/well of TEV Protease activity Fluorometric Detection
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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