Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)
CAT:
26-K698-100
Size:
100 Assays
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)
- Description: NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. BioVision’s Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.
- Reactivity: Mammalian samples. Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc.
- Applications: • Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells. • Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.
- Detection Method: Detection method- Absorbance (450 nm)
- Features & Benefits: Simple, colorimetric protocol. Detects ADH1-NADP activity as low as 0.001 mU in samples. Compatible with multiple sample types.
- Shipping Conditions: gel pack
- Storage Conditions: -20°C
- Shelf Life: 12 months