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Sphingomyelin Quantification Colorimetric Assay Kit

CAT: 0026-K600-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K600-100Size:100 Assays
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Description
Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.
Applications
• Sphingomyelin quantification in various biological samples • Analysis of cell signaling pathway
Detection Method
Absorbance (OD 570 nm)
Features & Benefits
• Simple, rapid & convenient • The assay can detect sphingomylein less than 1 µM in a variety of samples
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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