Sphingomyelin Quantification Colorimetric Assay Kit

CAT:

26-K600-100

Size:

100 Assays

Price:

Ask

For price, please contact [email protected]

Description: Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.
Applications: • Sphingomyelin quantification in various biological samples • Analysis of cell signaling pathway
Detection Method: Absorbance (OD 570 nm)
Features & Benefits: • Simple, rapid & convenient • The assay can detect sphingomylein less than 1 µM in a variety of samples
Shipping Conditions: gel pack
Storage Conditions: -20°C
Shelf Life: 12 months

Cat	Name
K600-100	Sphingomyelin Quantification Colorimetric Assay Kit
K253-25	DNA Damage Quantification Colorimetric Kit
K622-100	Triglyceride Quantification Colorimetric/Fluorometric Kit
K615-100	Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit
K612-100	Free Fatty Acid Quantification Colorimetric/Fluorometric Kit
K613-100	HDL and LDL/VLDL Quantification Colorimetric/Fluorometric Kit
K952-400	EZScreen™ Triglyceride Quantification Colorimetric Assay Kit (384-well)
K1202-100	ExoQuant™ Overall Exosome Capture and Quantification Assay Kit (Human Urine, Colorimetric)
K1203-100	ExoQuant™ Overall Exosome Capture and Quantification Assay Kit (Human Serum, Colorimetric)
K1205-100	ExoQuant™ Overall Exosome Capture and Quantification Assay Kit (Cell media, Colorimetric)

Cat	Name
K2100-25	DNA Damage Quantification Colorimetric Kit
K2127-100	Triglyceride Quantification Colorimetric/Fluorometric Kit
MBS168769-01	Serum Triglyceride Quantification Kit (Colorimetric)
MBS168769-02	Serum Triglyceride Quantification Kit (Colorimetric)
STA-396	Serum Triglyceride Quantification Kit (Colorimetric)
TBS2029	NADH/NAD Quantification Colorimetric Assay Kit
TBS2058	NADP/NADPH Quantification Colorimetric Assay Kit
K2123-100	Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit
P-3072-96	EpiQuik Total Histone H4 Quantification Kit (Colorimetric)
P-9005-48	EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)