CETP Activity Fluorometric Assay Kit II

CETP Activity Fluorometric Assay Kit II

• Description: Cholesteryl ester transfer protein (CETP) is a plasma protein that transfers a cholesteryl ester from HDL to LDL or VLDL in exchange for a triglyceride. HDL plays an important role in lipid metabolism and cardiovascular health. HDL transports cholesterol to the liver for excretion or to steroidogenic tissues for steroid synthesis. HDL also plays an important role in the reverse cholesterol transport pathway, removing cholesterol from lipid-filled macrophages, protecting against atherosclerosis. Because of this function, CETP is viewed as a target to increase HDL, with CETP inhibition an active area of research and several CETP inhibitors at various stages of drug development. BioVision’s CETP Activity Fluorometric Assay Kit uses a self-quenched fluorescent neutral lipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of neutral lipid transfer. Rabbit serum is provided as a positive control and CETP inhibitor Torcetrapib is included for assay validation. This Assay Kit, in addition to measuring activity in serum, is also suitable for testing activity of recombinant protein.
• Applications: Measurement of CETP activity in animal serum, plasma and recombinant protein
• Detection Method: Detection method- Fluorescence (Ex/Em = 480/511 nm)
• Features & Benefits: • Simple, rapid & convenient assay to measure CETP activity in animal serum, plasma or recombinant protein. • Includes Inhibitor Control, Torcetrapib to validate the assay. • Comes with Positive Control (rabbit serum).
• Shipping Conditions: gel pack
• Storage Conditions: 4°C
• Shelf Life: 12 months