Glyoxalase II Activity Kit (Colorimetric)

Glyoxalase II Activity Kit (Colorimetric)

• Description: Glyoxalase II (GloII, hydroxyacylglutathione hydrolase, EC 3.1.2.6) is one of the two enzymes in the Glyoxalase system that is ubiquitously expressed in mammalian, plants and bacteria. The Glyoxalase system is a biological pathway that detoxifies cells by metabolizing α-ketoaldehydes such as methylglyoxal, a cytotoxic byproduct of lipids and glucose metabolism. GloII fulfills the terminal step in the glyoxalase system: It catalyzes the hydrolysis of S-D-lactoyl-glutathione (SLG) to reduced glutathione and D-lactic acid. The alteration of GloII activity in cell growth cycle, cell differentiation, phagocyte activation and diabetes mellitus development of implies this enzyme plays a wide-ranging and important role in cell function and disease progression. Research areas of current interest in Glo-II include diabetes, and cancer therapy. Biovision’s Glyoxalase II Activity Kit utilizes the ability of an active Glo-II to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader. Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples
• Reactivity: Mammalian
• Applications: Measurmente of Glyoxalase II activity in biological samples
• Detection Method: Colorimetric (OD 450 nm)
• Features & Benefits: • Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of Glyoxalase II
• Shipping Conditions: gel pack
• Storage Conditions: -20°C
• Shelf Life: 12 months