Actin Polymerization/Depolymerization Assay Kit (Fluorometric)

• Description:

  Actin, a highly conserved and abundant protein in eukaryotic cells, is one of the major components of cytoskeleton. It can be found as monomeric globular protein, called G actin or it can polymerize into filamentous actin, named F actin. Actin plays major roles in cell division, cell motility, cell signaling, organelle movement, etc. Mammals have 6 isoforms of Actin, which can be divided into 3 classes, α, β and γ. Muscle Actin is α class and all other non-muscle actins belong to β and γ -classes. Understanding the effect of different drugs, proteins, etc. on Actin Polymerization and Depolymerization is very important for understanding cellular machinery, more importantly because Cytoskeleton is a very important target for cancer therapy. BioVision’s Actin Polymerization/Depolymerization Assay Kit can be used to study the effect of different compounds, proteins and tissue extracts on Actin polymerization and depolymerization. The kit utilizes a proprietary Pyrene-labeled Actin molecule that develops a higher fluorescent signal if it undergoes polymerization. The signal can be easily detected using a fluorescence microplate reader. The assay is simple, high- throughput compatible, and can be completed in less than three hours

• Reactivity:

  Not Applicable

• Applications:

  • Study and quantitate the effect of different compounds, proteins and tissue extracts on Actin polymerization and/or depolymerization • Evaluation of critical concentrations of actin polymerization in different conditions

• Detection Method:

  Fluorescence (Ex/Em: 365/410 nm)

• Shipping Conditions:

  gel pack

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months