Products for Research Use Only
RegisterLogin

Phosphate Assay Kit (Fluorometric)

CAT: 0026-K420-100Size: 100 AssaysDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0026-K420-100Size:100 Assays
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of its most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in a variety of samples. The newly designed and enhanced Phosphate Assay Kit provides a sensitive, easy and quick means of assessing Pi over a wide range of concentrations. In this assay, inorganic phosphate reacts with sucrose to produce glucose-1-phosphate in the presence of a proprietary enzyme. The glucose-1-phosphate is specifically oxidized to generate a product that reacts with the PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, phosphorylase, etc. from a variety of samples. Unlike other commercially available assays, this assay is not affected by the presence of glucose in samples. This kit can determine phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
Reactivity
Mammalian samples
Applications
Measure Phosphate concentrations between 2 µM and 10 µM, with a lower detection limit of approximately 100 pmol.
Detection Method
Ex/Em 535/587nm
Features & Benefits
• Simple procedure; takes only ~2 hours • Fast and convenient • The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples.
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

Related Products

CatalogName
K419-100PicoProbe™ Phosphate Fluorometric Assay Kit
K687-100PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit
K689-100PicoProbe™ Fructose-6-Phosphate Fluorometric Assay Kit
K673-100PicoProbe™ Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
K2009-100Pyridoxal 5’-phosphate (Vit B6) Fluorometric Assay Kit
K2005-100Lysine Assay Kit (Fluorometric)
K327-100Adenosine Assay Kit (Fluorometric)
K939-100Ornithine Assay Kit (Fluorometric)
K565-100Phosphatidylserine Assay Kit (Fluorometric)
K589-100Glycine Assay Kit (Fluorometric)

Alternative Products

CatalogName
K2076-100Phosphate Assay Kit (Fluorometric)
GWB-AXR232Phosphate Fluorometric Assay Kit
E-BC-F151Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
K2222-100Fructose-6-Phosphate Fluorometric Assay Kit
K2220-100Glucose-6-Phosphate Fluorometric Assay Kit
MBS2576274-01Glycerol-3-phosphate (G3P) Fluorometric Assay Kit
MET-5076Glycerol-3-Phosphate (G3P) Assay Kit (Fluorometric)
TBS2102FGlucose-6-Phosphate Dehydrogenase Activity Fluorometric Assay Kit
MBS2577865-01Fructose-6-Phosphate (F-6-P) Fluorometric Assay Kit
N0009-74NADP/NADPH Assay Kit (Nicotinamide Adenine Dinucleotide Phosphate), BioAssayTM, Fluorometric discontinued

Popular Products