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EGF (mouse) ELISA Kit

CAT: 0026-K4023-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K4023-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BioVision’s mouse EGF (Epidermal Growth Factor) ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody from rat specific for EGF coated on a 96-well plate. Standards (E. Coli, N977-R1029) and test samples are added to the wells and EGF present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse EGF captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 1 pg/ml.
CAS Number
7732-18-5
Reactivity
mouse
Applications
This ELISA kit is used for quantitative protein detection, establishing normal range etc.
Detection Method
Absorbance (450 nm)
Features & Benefits
• Easy, convenient and time-saving method to assay mouse EGF. • Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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