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Thrombin Inhibitor Screening Kit (Fluorometric)

CAT: 0026-K374-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K374-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Thrombin enzyme (Factor IIa) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin inhibitors are used as anticoagulants to prevent arterial and venous thrombosis. Some of these inhibitors are currently in clinical use while others are in clinical development. Biovision’s Thrombin Inhibitor Screening Kit utilizes the ability of Thrombin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 350/450 nm. In the presence of Thrombin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Thrombin.
Applications
• Screening potential inhibitors of thrombin • Characterize/study thrombin inhibitors
Detection Method
Fluorescence (Ex/Em = 350/450 nm)
Features & Benefits
• Rapid & simple • Reliable test for screening potential inhibitors of thrombin • Includes thrombin Inhibitor [PPACK Dihydrochloride]
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
12 months

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