Caspase-10 Colorimetric Assay Kit

CAT:

26-K125-200

Size:

200 Assays

Price:

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Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Features & Benefits: • Simple one-step procedure; takes only 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent Caspase activity.
Shipping Conditions: gel pack
Storage Conditions: -20°C
Shelf Life: 6-12 months

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