Caspase-3 Colorimetric Assay Kit

CAT:

26-K106-25

Size:

25 Assays

Price:

Ask

For price, please contact [email protected]

Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Features & Benefits: • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Shipping Conditions: gel pack
Storage Conditions: -20°C
Shelf Life: 12 months

Cat	Name
K106-400	Caspase-3 Colorimetric Assay Kit
K106-100	Caspase-3 Colorimetric Assay Kit
K106-25	Caspase-3 Colorimetric Assay Kit
K106-200	Caspase-3 Colorimetric Assay Kit
K111-100	Caspase-1 Colorimetric Assay Kit
K111-200	Caspase-1 Colorimetric Assay Kit
K111-400	Caspase-1 Colorimetric Assay Kit
K113-25	Caspase-8 Colorimetric Assay Kit
K113-400	Caspase-8 Colorimetric Assay Kit
K117-25	Caspase-2 Colorimetric Assay Kit

Cat	Name
K2008-25	Caspase-3 Colorimetric Assay Kit
K2008-400	Caspase-3 Colorimetric Assay Kit
MBS9718955-01	Caspase-3 Assay Kit (Colorimetric)
MBS9718955-03	Caspase-3 Assay Kit (Colorimetric)
TBS2030	Caspase-3 Colorimetric Assay kit
MBS9718955-02	Caspase-3 Assay Kit (Colorimetric)
55R-1270	Caspase 3 Colorimetric Assay Kit
K2008-100	Caspase-3 Colorimetric Assay Kit
K2008-200	Caspase-3 Colorimetric Assay Kit
MBS9718955-04	Caspase-3 Assay Kit (Colorimetric)