MMP-1 (Human) ELISA Kit

MMP-1 (Human) ELISA Kit

• Description: Matrix metalloproteinase-1 (MMP-1), also known as collagenase-1, fibroblast collagenase, and interstitial collagenase belong to the family of matrix metalloproteinases (MMPs). Cells that express MMP-1 include macrophages, monocytes, keratinocytes, and fibroblasts. These endopeptidases can breakdown extracellular matrices as well as interstitial collagen types I, II, III. MMP-1 plays a significant role in diseases such as cancer, rheumatoid arthritis, fibrotic and cardiovascular diseases and thus could serve as a therapeutic target in the treatment of human diseases. BioVision’s MMP-1 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of MMP-1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of MMP-1 in the sample or standard.
• CAS Number: 7732-18-5
• Reactivity: Human
• Applications: This ELISA kit is used for quantitative measurement of MMP-1 in serum, plasma, tissue homogenates and other biological fluids
• Detection Method: Absorbance (450 nm)
• Features & Benefits: Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10% Detection range: 78.125 - 5000 pg/ml Sensitivity: 46.875 pg/ml Recovery range: 85 - 105% for normal human serum and plasma samples This Sandwich ELISA is highly sensitive and highly specific for the detection of MMP-1 in human samples. There is no significant cross-reactivity or interference between MMP-1 and analogues
• Shipping Conditions: gel pack
• Storage Conditions: 4°C
• Shelf Life: 6 months