Murashige & Skoog (MS) Basal Medium w/ Vitamins

Murashige & Skoog (MS) Basal Medium w/ Vitamins

• Description: Murashige and Skoog Media, MS Media is a nutrient media used for the in vitro culture of plant cells, tissues, and organs. It was first developed in 1962 by Toshio Murashige and Folke Skoog and has since become one of the most widely used media for plant tissue culture. It contains a balanced mixture of macronutrients, micronutrients, vitamins, and plant growth regulators to provide optimal conditions for the proper growth and development of plants.
• Synonyms: MS Basal Medium
• HS Code: 3824.99
• Applications: In vitro plant growth and development Callus formation induction
• Assay Protocol: How To Use Murashige & Skoog Media Follow the given steps to prepare 1 liter of tissue culture media using MS media: Take a beaker and add 800 ml of distilled water to it. To the water add PCT’s MS Media 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L) Make up the volume of the media to 1000ml. Adjust the pH of the media between 5.4-5.8. Add gelling agent (3-4 g/L gellan gum or 6-8 g/L agar) to the media and keep stirring. Autoclave the medium for 20 minutes at 15 psi and 121°C. Your media is ready! Note: Media should be prepared according to the formula mentioned on the label. However, it is recommended to use an entire container at once. Heat-liable substrates should be added, after autoclaving.
• Capacity: Available in volumes of 5 L, 10 L, 50 L, and 100 L
• Format: Cylindrical container
• Material: Liquid
• Color: White to light tan
• Solubility: Partially soluble in cold water and completely soluble in hot water
• Features & Benefits: Easy to use Supply nutrients required by in vitro plants for their proper growth and development Provides a well-balanced composition of nutrients, including essential minerals and vitamins, supporting the growth of various plant tissues. Effective in inducing callus formation A dynamic supplier of numerous amino acids, organic acids, plant growth regulators (such as cytokinins and auxins), vitamins, sugars, minerals, nucleic acids, and unidentified growth-promoting substances.
• Storage Temperature: at 2-8 °C and away from direct light
• Shelf Life: See expiry date on the bottle
• Appearance: Homogenous mixture of free-flowing powder