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Nucleolin Antibody

CAT: 0013-GTR18574203Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18574203Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleolin (NCL) is the major nucleolar phosphoprotein of growing eukaryotic cells. It is located mainly in dense fibrillar regions of the nucleolus. NCL is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
UniProt
P19338
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clonality
Monoclonal
Clone
NPC23-2
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml, Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the Nucleolin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1

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