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Insulin Antibody

CAT: 0013-GTR18574105Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18574105Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. [RefSeq]
UniProt
P01308
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant INS protein was used as the immunogen for the Insulin antibody.
Clonality
Monoclonal
Clone
BCPH1
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Insulin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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