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Calponin Antibody

CAT: 0013-GTR18573969Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573969Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
UniProt
P51911
Reactivity
Human, Rat
Immunogen
Recombinant human protein was used as the immunogen for the Calponin antibody.
Clonality
Monoclonal
Clone
CLPN5-1
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · P51911

Calponin-1

CNN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P51911
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CNN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R868, B4DUX6, O00638, Q15416, Q8IY93, Q99438
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-bindingCalmodulin-binding

Coding sequence diversity

Alternative splicing

PTM

Phosphoprotein

Domain

Repeat

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