ZAP70 Antibody

CAT: 0013-GTR18573787Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18573787Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Tyrosine-protein kinase ZAP70 is a tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, it phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. [UniProt]

UniProt

P43403

Reactivity

Human

Immunogen

A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.

Clonality

Monoclonal

Clone

ZPT70-2

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the ZAP70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Tested Applications

FACS, IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

UniProtKB · P43403

Tyrosine-protein kinase ZAP-70

ZAP70_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P43403
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ZAP70
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.10.2
Processing: —
Secondary accessions: A6NFP4, Q6PIA4, Q8IXD6, Q9UBS6

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantSCID

Molecular function

KinaseTransferaseTyrosine-protein kinase

Domain

RepeatSH2 domain

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