Villin Antibody / VIL1

Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA) ; binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF) -induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]

P09327

Human

Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

VIL1/1325

Unconjugated

Cell Biology

Protein G affinity chromatography

Western blot: 1-2ug/ml for 60 min at RT, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

For research use only.

The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

IHC-P, WB

Mouse

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free