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E. coli O157 Antibody

CAT: 0013-GTR18573217Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573217Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
Reactivity
E. coli
Immunogen
A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.
Clonality
Monoclonal
Clone
9.88
Conjugation
Unconjugated
Field of Research
Infectious Disease & Virology
Purification
PEG precipitation followed by dialysis in 1X PBS
Dilution
ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the E. coli O157 antibody should be determined by the researcher.
Tested Applications
ELISA, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgM, kappa

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