Rabbit anti Human Sphingomyelin Synthase 1 (Peptide as antigen)

• Background:

  A bidirectional lipid cholinephosphotransferases capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and the inverse reaction. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. May not function strictly as a SM synthase. Inhibited by bacterial PC-phospholipase C inhibitor D609. Localized to Golgi apparatus; Golgi membrane; multi-pass membrane protein. Possibly present on Brain, heart, kidney, liver, muscle and stomach.

• Synonyms:

  Phosphatidylcholine:ceramide cholinephosphotransferase 1 Transmembrane Protein 23, Sphingomyelin synthase 1, Mob Protein

• UniProt:

  Q86VZ5

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Rabbits were immunized with a synthetic peptide derived from the mouse sphingomyelin synthase 1 protein

• Applications:

  ELISA, WB

• Field of Research:

  Signal Transduction

• Purification Method:

  High Salt Precipitation

• Assay Principle:

  Antibody can be used for Western blotting (5-10 µg/mL). Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Huitema K, van den Dikkenberg J, Brouwers JF, Holthuis JC Identification of a family of animal sphingomyelin synthases. EMBO J. 2004 Jan 14;23(1):33-44.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4