Products for Research Use Only
RegisterLogin

CD8a Antibody Cocktail

CAT: 0013-GTR18573088Size: 7 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18573088Size:7 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8a/b) or as a homodimer (CD8a/a) . A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
UniProt
P01732
Reactivity
Human
Immunogen
Human recombinant protein (C8/468) and 13 amino acid peptide from the C-terminal cytoplasmic domain of the alpha chain of human CD8 (C8/144B) were used as the immunogens for this CD8a antibody cocktail.
Clonality
Monoclonal
Clone
C8/468 + C8/144B
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The optimal dilution of the CD8a antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18571765CD8A Antibody / CD8 alpha
GTR18089746-01CD8A Antibody / CD8 alpha
GTR18225355CD8A/CD8 Alpha Antibody
GTR18573512MITF Antibody Cocktail
GTR18573282PAX8 Antibody Cocktail
GTR18573097Myogenin Antibody Cocktail
GTR18572605SMMHC Antibody Cocktail
GTR18572397CD57 Antibody Cocktail
GTR18572387GFAP Antibody Cocktail
GTR18572386GFAP Antibody Cocktail

Alternative Products

CatalogName
V3134IHC-7MLCD8a Antibody Cocktail
RD30551F-01Anti-Human CD3-APC/CD4-FITC/CD8a-PE Cocktail
E-AB-FC0002-01Anti-Human CD3-APC/CD4-FITC/CD8a-PerCP-Cyanine5.5 Cocktail
V3139-20UGMyogenin Antibody Cocktail
V2027IHC-7MLCD1a Antibody Cocktail
V3156SAF-100UGMelan-A Antibody Cocktail
V2694SAF-100UGEpCAM Antibody Cocktail
V2455IHC-7MLChromogranin A Antibody Cocktail
V2937-20UGZAP70 Antibody Cocktail
V2752SAF-100UGSMMHC Antibody Cocktail

Popular Products