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BrdU Antibody

CAT: 0013-GTR18573055Size: 7 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18573055Size:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Reactivity
All
Immunogen
Bromodeoxyuridine conjugated to BSA was used as the immunogen for the BrdU antibody.
Clonality
Monoclonal
Clone
BRD.3
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the BrdU antibody should be determined by the researcher.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

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