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HCAM Antibody

CAT: 0013-GTR18572920Size: 7 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18572920Size:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
UniProt
P16070
Reactivity
Human, Mouse
Immunogen
Recombinant human protein was used as the immunogen for the HCAM antibody.
Clonality
Monoclonal
Clone
HCAM/1097
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the HCAM antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

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