ZAP70 Antibody

CAT: 0013-GTR18572854Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18572854Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

UniProt

P43403

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the ZAP70 antibody.

Clonality

Monoclonal

Clone

ZAP70/528

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the ZAP70 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG2a, kappa

UniProtKB · P43403

Tyrosine-protein kinase ZAP-70

ZAP70_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P43403
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ZAP70
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.10.2
Processing: —
Secondary accessions: A6NFP4, Q6PIA4, Q8IXD6, Q9UBS6

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantSCID

Molecular function

KinaseTransferaseTyrosine-protein kinase

Domain

RepeatSH2 domain

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