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VEGF Antibody / VEGFA

CAT: 0013-GTR18572833Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572833Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
UniProt
P15692
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the VEGF antibody.
Clonality
Monoclonal
Clone
VEGF/1063
Conjugation
Unconjugated
Field of Research
Cardiovascular Research; Cell Biology
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the VEGF antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

UniProtKB · P15692

Vascular endothelial growth factor A, long form

VEGFA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15692
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VEGFA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
L-VEGF
EC number
—
Processing
Precursor
Secondary accessions
B5BU86, H0Y2S8, H0Y407, H0Y414, H0Y462, H0Y8N2, H3BLW7, O60720, O75875, Q074Z4, Q16889, Q5UB46, Q6P0P5, Q96KJ0, Q96L82, Q96NW5, Q9H1W8, Q9H1W9, Q9UH58, Q9UL23
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative initiationAlternative promoter usageAlternative splicing

Biological process

AngiogenesisDifferentiation

Cellular component

CytoplasmEndoplasmic reticulumExtracellular matrixGolgi apparatusNucleusSecreted

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Disulfide bondGlycoprotein

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