Products for Research Use Only
RegisterLogin

CD43 Antibody

CAT: 0013-GTR18572758Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18572758Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
UniProt
P16150
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the CD43 antibody.
Clonality
Monoclonal
Clone
SPN/839
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD43 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18212797-01CD43 monoclonal antibody
GTR18571274-01SPN Antibody / CD43
GTR18571273SPN Antibody / CD43
GTR18212860-01CD43 monoclonal antibody
GTR18714651-01CD43 Mouse mAb Antibody
GTR18221351CD43 (Clone MT1) Antibody
GTR18293374CD43 (T-Cell) Antibody
GTR18293373CD43, T-Cell Antibody
GTR18292617CD43 (T-Cell) Antibody
GTR18292082CD43 (T-Cell) Antibody

Alternative Products

CatalogName
W0282-01CD43 (ABT-CD43) Antibody
MBS9510632-01Anti-CD43 Antibody [ABT-CD43]
10R-6398CD43 antibody
GWB-53CED1CD43, Antibody

Popular Products