Products for Research Use Only
RegisterLogin

Nucleolin Antibody

CAT: 0013-GTR18572611Size: 7 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18572611Size:7 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL) . It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
UniProt
P19338
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the anti-Nucleolin antibody.
Clonality
Monoclonal
Clone
SPM614
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the anti-Nucleolin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18292297Nucleolin (Marker of Nucleoli) Antibody
GTR18292936Nucleolin (Marker of Nucleoli) Antibody
GTR18273913Nucleolin Conjugated Antibody
GTR18572612NCL Antibody / Nucleolin
GTR18298121-01Nucleolin monoclonal antibody
GTR18219981-01Rabbit Nucleolin (NCL) Antibody
GTR18219978-01Rabbit Nucleolin (NCL) Antibody
GTR18107915-01Nucleolin Rabbit Polyclonal Antibody
GTR18207688-01Nucleolin Rabbit mAb Antibody
GTR18710312Nucleolin Rabbit mAb Antibody

Alternative Products

CatalogName
MBS9435026-01Nucleolin Antibody

Popular Products