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Mucin-6 Antibody

CAT: 0013-GTR18572585Size: 7 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18572585Size:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MUC6 or gastric mucin is a secreted glycoprotein that plays an essential role in epithelial cyto-protection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileum and right colon. In gastric cancer, Mucin 6 has an altered expression. In normal stomach, Mucin 6 is associated with Lewis type 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas. Mucin 6 is a secretory mucin, located in the deeper mucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
UniProt
Q6W4X9
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the Mucin-6 antibody.
Clonality
Monoclonal
Clone
MUC6/916
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Mucin-6 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

UniProtKB · Q6W4X9

Mucin-6

MUC6_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6W4X9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MUC6
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MUC-6
EC number
—
Processing
Precursor
Secondary accessions
O15329, Q14394, Q2TUQ5, Q4L207, Q8N8I1, Q8NAK1
Protein keywords

PTM

Disulfide bondGlycoprotein

Technical term

Proteomics identificationReference proteome

Domain

RepeatSignal

Cellular component

Secreted

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