Lactadherin Antibody

CAT: 0013-GTR18573368Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18573368Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-EA. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

UniProt

Q08431

Reactivity

Human

Immunogen

A delipidated human milk fat globule membrane preparation was used as the immunogen for the Lactadherin antibody.

Clonality

Monoclonal

Clone

EDM45

Conjugation

Unconjugated

Field of Research

Cardiovascular Research

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Lactadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, kappa

UniProtKB · Q08431

Lactadherin

MFGM_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q08431
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MFGE8
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: B2R6M7, F5GZN3, Q53FU9, Q7Z3D2, Q9BTL9

Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

AmyloidCytoplasmic vesicleMembraneSecreted

Biological process

AngiogenesisCell adhesionFertilization

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

EGF-like domainRepeatSignal

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