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Keratin 7 Antibody

CAT: 0013-GTR18572547Size: 7 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18572547Size:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-) .
UniProt
P08729
Reactivity
Human
Immunogen
OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Keratin 7 antibody.
Clonality
Monoclonal
Clone
SPM270
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Keratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Isotype
Mouse IgG1, kappa

UniProtKB · P08729

Keratin, type II cytoskeletal 7

K2C7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08729
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q92676, Q9BUD8, Q9Y3R7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondMethylationPhosphoproteinUbl conjugation

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filamentKeratin

Biological process

Host-virus interaction

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