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CD15 Antibody

CAT: 0013-GTR18572375Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572375Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
UniProt
P22083
Reactivity
Human
Immunogen
Myelomonocytic leukemia cells were used as the immunogen for the CD15 antibody.
Clonality
Monoclonal
Clone
BRA-4F1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
PEG precipitation
Dilution
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD15 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgM, kappa

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