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CD8 Antibody (alpha)

CAT: 0013-GTR18572330Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572330Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.
UniProt
P01732
Reactivity
Human
Immunogen
Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.
Clonality
Monoclonal
Clone
C8/1035
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/million cells
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.
Tested Applications
ELISA, FACS
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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