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HER2 Antibody ErbB2

CAT: 0013-GTR18572322Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18572322Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Reactivity
Human
Immunogen
Recombinant human HER2 protein was used as the immunogen for this antibody. (1)
Clonality
Monoclonal
Clone
HRB2/273
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Dilution
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. This HER2 antibody binds to the extracellular/cell surface region of the protein.
Tested Applications
ELISA, FACS, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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