Calponin Antibody

CAT: 0013-GTR18572193Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18572193Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

UniProt

P51911

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the anti-Calponin antibody.

Clonality

Monoclonal

Clone

CNN1/832

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the anti-Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG1, kappa

UniProtKB · P51911

Calponin-1

CNN1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P51911
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CNN1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: B2R868, B4DUX6, O00638, Q15416, Q8IY93, Q99438

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Molecular function

Actin-bindingCalmodulin-binding

Coding sequence diversity

Alternative splicing

PTM

Phosphoprotein

Domain

Repeat

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