Products for Research Use Only
RegisterLogin

CD63 Antibody / LAMP-3

CAT: 0013-GTR18571864Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18571864Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Reactivity
Human, Mouse
Immunogen
The smooth plasma membrane fraction of MeWo cells was used as the immunogen for this CD63 antibody.
Clonality
Monoclonal
Clone
NKI/C3
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G purified antibody
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR18572937LAMP-3 Antibody / CD63
GTR18082446-01LAMP-3 Antibody / CD63
GTR18572934LAMP-3 Antibody / CD63
GTR18082435-01LAMP-3 Antibody / CD63
GTR18572936LAMP-3 Antibody / CD63
GTR18571669-01Recombinant LAMP-3 Antibody / CD63
GTR18571658Recombinant LAMP-3 Antibody / CD63
GTR18317516-01Recombinant LAMP-3 Antibody / CD63
GTR18317515Recombinant LAMP-3 Antibody / CD63
GTR18997897LAMP-3/DC-LAMP/CD208 Antibody Blocking Peptide

Alternative Products

CatalogName
V2070-100UGCD63 Antibody / LAMP-3
V3028-100UGLAMP-3 Antibody / CD63

Popular Products