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CD34 Antibody

CAT: 0013-GTR18571856Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18571856Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi’s sarcoma, but shows low specificity.
Reactivity
Human, Rat
Immunogen
Blast cells from a chronic myeloid leukemia patient were used as the immunogen for this CD34 antibody.
Clonality
Monoclonal
Clone
ICO-115
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Tested Applications
ELISA, FACS, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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