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CD28 Antibody

CAT: 0013-GTR18571849Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18571849Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical Tcell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing Tcells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. it is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for this CD28 antibody.
Clonality
Monoclonal
Clone
CB28
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Functional studies (order BSA/sodium azide-free format)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD28 antibody to be titered up or down for optimal performance.
Tested Applications
FACS, Functional Studies, IF
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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