CD5 Antibody

CAT: 0013-GTR18571838Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18571838Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This antibody recognizes a 67kDa transmembrane protein, which is identified as CD5. It is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-) . It does not react with granulocytes or monocytes.

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for this CD5 antibody.

Clonality

Monoclonal

Clone

CRIS-1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Functional studies (order BSA/sodium azide-free format), Immunoprecipitation (0.5-1ug/500ug protein lysate)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD5 antibody to be titered up or down for optimal performance.

Tested Applications

FACS, Functional Studies, IF, IP

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

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