Products for Research Use Only
RegisterLogin

Bax Antibody

CAT: 0013-GTR18571800Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18571800Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This antibody recognizes a protein of 21kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Reactivity
Human
Immunogen
Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.
Clonality
Monoclonal
Clone
2D2
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 2-4ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

Related Products

CatalogName
GTR17693487Apoptosis regulator BAX Bax Rabbit Polyclonal Antibody
GTR18064809-01Bax Polyclonal Antibody
GTR18301433-01Bax monoclonal antibody
GTR18294718-01Bax polyclonal antibody
GTR18290958-01BAX monoclonal antibody
GTR18057069-01Bax (Ab67) Antibody
GTR18299932-01Bax monoclonal antibody
GTR18294824-01Bax polyclonal antibody
GTR18290504-01BAX monoclonal antibody
GTR18199001Bax Polyclonal Antibody

Alternative Products

CatalogName
MBS8529199-01H2B Antibody Antibody
MBS8513599-01CD11b Antibody Antibody
DF8746-01ASAP1 antibody Antibody
MBS850197-01Antibody to IGFBP2 Antibody
E36403ZNF98 Antibody Antibody
MBS9403836-01SZT2 Antibody Antibody
GWB-250026Lck antibody Antibody
E8M1007-4HSP60 Antibody Antibody
LSMab09882anti-Antibody^Polyclonal antibody control antibody
GWB-5C6547MAD1L1 antibody [9B10] Antibody

Popular Products