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Recombinant Vimentin Antibody

CAT: 0013-GTR18571612Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18571612Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Anti-Vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
UniProt
P08670
Reactivity
Bovine, Canine, Equine, Feline, Human, Mouse, Rat
Immunogen
A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant Vimentin antibody.
Clonality
Recombinant
Clone
VIM/6430R
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Vimentin antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG

UniProtKB · P08670

Vimentin

VIME_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08670
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B0YJC2, D3DRU4, Q15867, Q15868, Q15869, Q548L2, Q6LER9, Q8N850, Q96ML2, Q9NTM3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinIsopeptide bondPhosphoproteinS-nitrosylationUbl conjugation

Disease

CataractDisease variant

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonIntermediate filamentMembraneNucleus

Domain

Coiled coil

Biological process

Host-virus interaction

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