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CD73 Antibody

CAT: 0013-GTR18574619Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18574619Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]
UniProt
P21589
Reactivity
Human
Immunogen
A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Clonality
Monoclonal
Clone
CDLA73-1
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
ELISA: order BSA-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the CD73 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
ELISA, FACS, IF, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG1, kappa

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