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BOB1 Antibody

CAT: 0013-GTR18574372Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18574372Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
UniProt
Q16633
Reactivity
Human
Immunogen
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.
Clonality
Monoclonal
Clone
BOB1/2421
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein G affinity
Dilution
Flow cytometry: 1-2ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the BOB1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FACS, IHC-P, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa

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