Nucleophosmin Antibody

CAT: 0013-GTR18574364Size: 7 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18574364Size:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM) . It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

UniProt

P06748

Reactivity

Human

Immunogen

A portion of amino acids 185-287 from the human protein was used as the immunogen for the Nucleophosmin antibody.

Clonality

Monoclonal

Clone

NPM1/1902

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Nucleophosmin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG2b, kappa

UniProtKB · P06748

Nucleophosmin

NPM_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P06748
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: NPM1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: NPM
EC number: —
Processing: —
Secondary accessions: A8K3N7, B5BU00, D3DQL6, P08693, Q12826, Q13440, Q13441, Q14115, Q5EU94, Q5EU95, Q5EU96, Q5EU97, Q5EU98, Q5EU99, Q6V962, Q8WTW5, Q96AT6, Q96DC4, Q96EA5, Q9BYG9, Q9UDJ7

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationDisulfide bondIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Molecular function

ChaperoneRNA-binding

Cellular component

CytoplasmCytoskeletonNucleus

Biological process

Host-virus interaction

Disease

Proto-oncogene

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Alternative Products

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70R-50146	Nucleophosmin antibody