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Ep-CAM Antibody

CAT: 0013-GTR18573319Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18573319Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
UniProt
P16422
Reactivity
Canine, Feline, Human
Immunogen
A human partial recombinant protein corresponding to amino acids 100-224 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.
Clonality
Monoclonal
Clone
EGP40/1798
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
ELISA: 2-4ug/ml (order BSA/azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the Ep-CAM antibody may be required for optimal performance.
Tested Applications
ELISA, FACS, IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa

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